| 000 | 05548nam a2200721 i 4500 | ||
|---|---|---|---|
| 001 | 9780750330398 | ||
| 003 | IOP | ||
| 005 | 20230516170223.0 | ||
| 006 | m eo d | ||
| 007 | cr cn |||m|||a | ||
| 008 | 210611s2021 enka fob 000 0 eng d | ||
| 020 |
_a9780750330398 _qebook |
||
| 020 |
_a9780750330381 _qmobi |
||
| 020 |
_z9780750330374 _qprint |
||
| 020 |
_z9780750330404 _qmyPrint |
||
| 024 | 7 |
_a10.1088/978-0-7503-3039-8 _2doi |
|
| 035 | _a(CaBNVSL)thg00082502 | ||
| 035 | _a(OCoLC)1259501142 | ||
| 040 |
_aCaBNVSL _beng _erda _cCaBNVSL _dCaBNVSL |
||
| 050 | 4 |
_aQP801.P64 _bS567 2021eb |
|
| 072 | 7 |
_aPHVN _2bicssc |
|
| 072 | 7 |
_aSCI009000 _2bisacsh |
|
| 082 | 0 | 4 |
_a574.19/283 _223 |
| 245 | 0 | 0 |
_aSingle-particle cryo-EM of biological macromolecules / _cedited by Robert M. Glaeser, Eva Nogales, Wah Chiu. |
| 264 | 1 |
_aBristol [England] (Temple Circus, Temple Way, Bristol BS1 6HG, UK) : _bIOP Publishing, _c[2021] |
|
| 300 |
_a1 online resource (various pagings) : _billustrations (some color). |
||
| 336 |
_atext _2rdacontent |
||
| 337 |
_aelectronic _2isbdmedia |
||
| 338 |
_aonline resource _2rdacarrier |
||
| 490 | 1 | _a[IOP release $release] | |
| 490 | 1 | _aBiophysical Society-IOP series | |
| 490 | 1 | _aIOP ebooks. [2021 collection] | |
| 500 | _a"Version: 20210205"--Title page verso. | ||
| 504 | _aIncludes bibliographical references. | ||
| 505 | 0 | _a1. Introduction and overview -- 1.1. Visualizing biological molecules to understand life's principles -- 1.2. Recovery of 3D structures from images of weak-phase objects | |
| 505 | 8 | _a2. Sample preparation -- 2.1. Overview -- 2.2. Initial screening of samples in negative stain -- 2.3. Standard method of making grids for cryo-EM -- 2.4. Requirement to make very thin specimens for cryo-EM -- 2.5. Current strategies for optimizing preparation of cryo-grids | |
| 505 | 8 | _a3. Data collection -- 3.1. Overview -- 3.2. Radiation damage in cryo-EM -- 3.3. Low-dose protocols for recording images -- 3.4. Practical considerations : defocus, stigmation, coma-free illumination, and phase plates -- 3.5. Practical considerations : movie-mode data acquisition | |
| 505 | 8 | _a4. Data processing -- 4.1. Overview -- 4.2. Automated extraction of particles -- 4.3. CTF estimation and image correction (restoration) -- 4.4. Merging data from structurally homogeneous subsets -- 4.5. 3D classification of structurally heterogeneous particles -- 4.6. Preferred orientation : how to recognize and deal with adverse effects -- 4.7. B factors and map sharpening -- 4.8. Optical aberrations and Ewald sphere curvature | |
| 505 | 8 | _a5. Map validation -- 5.1. Overview -- 5.2. Measures of resolution : FSC and local resolution -- 5.3. Recognizing the effect of bias and over-fitting -- 5.4. Estimates of alignment accuracy -- 5.5. Discussion | |
| 505 | 8 | _a6. Model building and validation -- 6.1. Overview -- 6.2. Using known components or homologs : model building -- 6.3. Building atomistic models in cryo-EM density maps -- 6.4. Quality evaluation of cryo-EM map-derived models -- 6.5. How algorithms from crystallography are helping electron cryo-microscopy -- 6.6. Archiving structures and data. | |
| 520 | 3 | _aThis edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. It is especially well suited for larger complexes and molecular machines. This book provides a comprehensive, accessible and authoritative introduction to the field. It covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data collection, data analysis, and the final validation of results. | |
| 521 | _aBeginning scientists in academia, pharma, and biotech. | ||
| 530 | _aAlso available in print. | ||
| 538 | _aMode of access: World Wide Web. | ||
| 538 | _aSystem requirements: Adobe Acrobat Reader, EPUB reader, or Kindle reader. | ||
| 545 | _aProfessor Glaeser (UC Berkeley) made major contributions to the initial development of cryo-EM technology, and he currently is active in the improvement of specimen preparation. Professor Nogales (UC Berkeley) has used cryo-EM throughout her scientific career, successfully applying this technique for the last three decades to systems that defied structural characterization. Professor Chiu (Stanford University) developed early experimental and computational methods toward 3D structure determination of biomolecules toward atomic resolution. | ||
| 588 | 0 | _aTitle from PDF title page (viewed on June 11, 2021). | |
| 650 | 0 |
_aMacromolecules _xAnalysis. _970271 |
|
| 650 | 0 |
_aBiomolecules _xAnalysis. _912076 |
|
| 650 | 0 |
_aHigh resolution imaging. _911219 |
|
| 650 | 7 |
_aBiophysics. _2bicssc _94093 |
|
| 650 | 7 |
_aSCIENCE / Life Sciences / Biophysics. _2bisacsh _914896 |
|
| 700 | 1 |
_aGlaeser, Robert M., _eauthor. _970272 |
|
| 700 | 1 |
_aNogales, Eva, _eauthor. _970273 |
|
| 700 | 1 |
_aChiu, Wah, _eauthor. _970274 |
|
| 710 | 2 |
_aInstitute of Physics (Great Britain), _epublisher. _911622 |
|
| 776 | 0 | 8 |
_iPrint version: _z9780750330374 _z9780750330404 |
| 830 | 0 |
_aIOP (Series). _pRelease 21. _970275 |
|
| 830 | 0 |
_aBiophysical Society-IOP series. _970276 |
|
| 830 | 0 |
_aIOP ebooks. _p2021 collection. _970277 |
|
| 856 | 4 | 0 | _uhttps://iopscience.iop.org/book/978-0-7503-3039-8 |
| 942 | _cEBK | ||
| 999 |
_c82810 _d82810 |
||