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024 7 _a10.1088/978-0-7503-3115-9
_2doi
035 _a(CaBNVSL)thg00082582
035 _a(OCoLC)1266548338
040 _aCaBNVSL
_beng
_erda
_cCaBNVSL
_dCaBNVSL
050 4 _aRC262
_b.M547 2021eb vol. 3
060 4 _aQZ 203
_bM632p 2021eb vol. 3
072 7 _aPHVN
_2bicssc
072 7 _aSCI009000
_2bisacsh
082 0 4 _a616.994
_223
100 1 _aMierke, Claudia Tanja,
_eauthor.
_970303
245 1 0 _aPhysics of cancer.
_nVolume 3,
_pExperimental biophysical techniques in cancer research /
_cClaudia Tanja Mierke.
246 3 0 _aExperimental biophysical techniques in cancer research.
250 _aSecond edition.
264 1 _aBristol [England] (Temple Circus, Temple Way, Bristol BS1 6HG, UK) :
_bIOP Publishing,
_c[2021]
300 _a1 online resource (various pagings) :
_billustrations (some color).
336 _atext
_2rdacontent
337 _aelectronic
_2isbdmedia
338 _aonline resource
_2rdacarrier
490 1 _a[IOP release $release]
490 1 _aBiophysical Society-IOP series
490 1 _aIOP ebooks. [2021 collection]
500 _a"Version: 202108"--Title page verso.
504 _aIncludes bibliographical references.
505 0 _a1. Introduction to cell and matrix mechanics and experimental techniques -- 1.1. Introduction to cell migration and invasion -- 1.2. The effect of heterogeneity of cells and their microenvironments -- 1.3. Cell protuberances change the microenvironment during migration and invasion -- 1.4. Traditional migration and invasion assays with adjustable matrix structure and mechanics -- 1.5. Changing cell mechanics to control cell migration and invasion -- 1.6. Mechanotransduction leads to cell differentiation and governs cell functions -- 1.7. Future directions of cell mechanics driving cell motility
505 8 _a2. 2D and 3D migration and invasion in pure and composite matrices -- 2.1. 2D migration assays -- 2.2. 2D collective migration and mechanical waves -- 2.3. 3D invasion assays with tunable structural and structure-based mechanical environmental cues -- 2.4. Composite gels of alginate and Matrigels -- 2.5. Composite gels of Matrigel and collagen -- 2.6. Matrigel increases the pore size and heterogeneity of the meshwork -- 2.7. Advantages and disadvantages of cell migration and invasion assays -- 2.8. Future focus of single-cell migration and invasion
505 8 _a3. Mechano-invasion assays : mechanical excitation enhances cell migration and invasion -- 3.1. Introduction to the mechanical excitation of cells -- 3.2. Mechano-invasion assays -- 3.3. Cyclic mechanical strain -- 3.4. Indirect effect of myosin contractility on cancer cells -- 3.5. Directional motility is based on mechanical cues -- 3.6. Compressive stress on cancer cells -- 3.7. How can cells sense mechanical cues? -- 3.8. Discussion of the advantages and disadvantages of mechano-invasion assays -- 3.9. The future directions of mechano-invasion approaches
505 8 _a4. Atomic force microscopy probing for cell, nucleus and matrix mechanics -- 4.1. Introduction to atomic force microscopy -- 4.2. Analysis of cell stiffness -- 4.3. What are the optimal settings for the investigation of cell mechanics employing AFM? -- 4.4. Calibration for force measurement using AFM -- 4.5. Force curves and data processing with AFM -- 4.6. Contact mechanics models commonly employed for cell mechanics analysis -- 4.7. Cell mechanics employing AFM -- 4.8. Analysis of intracellular structures, including organelles -- 4.9. Mapping stiffness of intracellular components in fixed cells -- 4.10. Analysis of matrix mechanics in the presence and absence of cells -- 4.11. Determination of cell-cell adhesion forces -- 4.12. Spontaneous oscillations between cell adhesion forces and stiffness -- 4.13. Analysis of cell-matrix adhesion forces -- 4.14. Discussion of the strengths and weaknesses of AFM
505 8 _a5. The micropipette aspiration technique -- 5.1. Introduction to micropipette aspiration techniques for living cells -- 5.2. Principles of capillary actin inside the micropipette capillary -- 5.3. Classical cell shape analysis with the micropipette aspiration technique -- 5.4. General biomechanical models -- 5.5. Cell-matrix adhesion force measurements involve pulling and pushing forces -- 5.6. Cell-substrate rupture forces using micropipette aspiration -- 5.7. Cell-cell detachment force measurements using a dual pipette approach -- 5.8. Nuclear mechanics -- 5.9. Discussion of the urgent need for novel and improved experimental techniques -- 5.10. Strengths, weaknesses and future of the micropipette aspiration technique
505 8 _a6. Traction force microscopy -- 6.1. Introduction to cellular forces -- 6.2. 2D forces on planar substrates -- 6.3. 3D forces in 3D extracellular matrix scaffolds of various matrix types -- 6.4. 3D forces with a mixed Matrigel-collagen type I (rat) matrix scaffold -- 6.5. How far can cells feel and sense the mechanics of substrates? -- 6.6. Analysis of the hypothesis stating that long sensing distances are based on strain stiffening -- 6.7. 3D traction force of single cells in collagen matrices -- 6.8. Forces of cells on fibrin matrices -- 6.9. The long-distance mechanical interaction of cells in 3D fibrin matrices -- 6.10. Forces of cells in 3D in the process of cell division -- 6.11. Measurement of intracellular forces at cell-cell junctions employing intracellular force and monolayer stress microscopy
505 8 _a7. Plate rheometer and atomic force microscopy are employed for 3D matrix mechanics characterization -- 7.1. Introduction to plate rheology -- 7.2. Stress relaxation of collagen hydrogels -- 7.3. Modification of collagen fibrils through bundling using chemicals in 3D biomimetic matrices -- 7.4. Collagen type I self-assembly and the role of D-spacing -- 7.5. Mechanical cues in the creation of aligned collagen fibers -- 7.6. Methodological challenges -- 7.7. Why are collagen matrices still state-of-the art for the modeling of cellular microenvironments?
505 8 _a8. Impedance measurements of living matter -- 8.1. Introduction to impedance measurements of material -- 8.2. Impedance measurement of living matter or tissues -- 8.3. Impedance measurements of malignant cancer tissue -- 8.4. Cells are measured using electrical impedance techniques -- 8.5. The electrode-electrolyte electrical model for cell measurements with impedance -- 8.6. Finite element model (FEM) -- 8.7. Characterization of cancer cell lines with the impedance technique -- 8.8. Electrical impedance analysis of living cells due to external cues -- 8.9. Impedance spectroscopy as a technique for capacity surveillance in 3D models of epithelial tissues -- 8.10. The current situation and future approaches -- 8.11. 3D cell cluster analysis using a multiplexed four-terminal (4T) impedance technique
505 8 _a9. Cyclic stretching of cells through substrate stretching -- 9.1. Introduction to cyclic stretching of cells -- 9.2. Principle and set-up of the cyclic cell stretcher -- 9.3. Cell reorientation cannot be predicted by previous theoretical approaches -- 9.4. A novel theory for the reorientation of cells -- 9.5. Does the theory match the experimental approach? -- 9.6. Low-cost version of a cyclic uniaxial cell stretcher -- 9.7. The reorientation and differentiation of cell phenotypes in 3D matrices -- 9.8. Breakdown of collagen scaffolds in the course of cell stretching -- 9.9. What function do boundaries in the alignment of cells due to stretch fulfill? -- 9.10. What are the mechanical features of cell alignment in 2D and 3D? -- 9.11. Engineering and population of collagen matrices with fibroblasts -- 9.12. The benefits and drawbacks of the cyclic cell stretching device.
520 3 _aThis is the third volume in the Physics of Cancer (Second Edition) set. The book introduces and discusses novel and advanced applications for probing and sensing mechanical properties of cells and their microenvironment. In addition, biophysical techniques for analysing combined biophysical techniques and cellular functions are presented and discussed. However, the major scope of the book is the presentation of biophysical approaches in cancer and its major findings that contribute significantly to the field of physics of cancer from a biophysical point of view. Each chapter is a closed unit and at its end references allow the reader to step more deeply into each topic. When possible, videos will be provided as a special feature to enhance the topics. Part of Biophysical Society-IOP series.
521 _aStudents, PhDs, Post-Docs, Professors, and Researchers in biophysics.
530 _aAlso available in print.
538 _aMode of access: World Wide Web.
538 _aSystem requirements: Adobe Acrobat Reader, EPUB reader, or Kindle reader.
545 _aClaudia Tanja Mierke is a full professor at the Peter Debye Institute for Soft Matter Physics and Head of the Department of Biological Physics at the University of Leipzig. Her main areas of research have included the interaction of cancer cells and endothelial cells; effects of external mechanical stimulation of cancer cells on gene expression and tissue invasiveness on different mechanical and structural environments; the investigation of the dynamic interplay between the cells and their microenvironment from a biophysical point of view.
588 0 _aTitle from PDF title page (viewed on September 1, 2021).
650 0 _aCancer cells
_xMechanical properties.
_970304
650 0 _aCancer.
_915213
650 0 _aBiophysics.
_94093
650 0 _aPathology, Molecular.
_914540
650 1 2 _aCell Transformation, Neoplastic.
_970305
650 2 2 _aCell Physiological Phenomena.
_970306
650 2 2 _aTumor Microenvironment.
_970307
650 2 2 _aBiomechanical Phenomena.
_970308
650 7 _aBiophysics.
_2bicssc
_94093
650 7 _aMedical physics and biophysics.
_2bisacsh
_970121
710 2 _aInstitute of Physics (Great Britain),
_epublisher.
_911622
776 0 8 _iPrint version:
_z9780750331135
_z9780750331166
830 0 _aIOP (Series).
_pRelease 21.
_970309
830 0 _aBiophysical Society-IOP series.
_970276
830 0 _aIOP ebooks.
_p2021 collection.
_970310
856 4 0 _uhttps://iopscience.iop.org/book/978-0-7503-3115-9
942 _cEBK
999 _c82815
_d82815